Recombinant Human IL-7 Protein (carrier free)

Product Specifications
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
Activity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530).   Specific activity is for reference purposes only and is not routinely tested.
Source: E. coli-derived human IL-7 protein
Asp26-His177, with an N-terminal Met
Accession #: P13232
N-terminal Sequence Analysis: Met
Predicted Molecular Mass :17 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution: Reconstitute 5 μg vials at 50 μg/mL in sterile PBS. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.