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  • Advanced Biomatrix Bovine Collagen Solution 5005-100ml
Advanced Biomatrix Bovine Collagen Solution 5005-100ml

Advanced Biomatrix Bovine Collagen Solution 5005-100ml

  • SKU: 5005-100ML
  • Price: USD$400
  • Product description: PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sou
  • Email Inquiry:info@equl.com

PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.



Coating Procedure


Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
  2. Dilute PureCol® in water to ~50 to 100 µg/ml (~1:30). A 0.01 M HCl solution may also be used.
  3. Swirl contents gently until material is completely mixed.
  4. Add appropriate amount of diluted PureCol® material to the culture surface ensuring that the entire surface is coated.
  5. Incubate at room temperature or 37°C, covered, for 1-2 hours.
  6. After incubation, aspirate any remaining material.
  7. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
  8. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

3-D Gel Preparation Procedure

  1. Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
  2. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
  3. Adjust final volume to a total of 10 parts with sterile water.
  4. To prevent gelation, maintain temperature of mixture at 2–10°C.
  5. To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.