The Total Dietary Fiber test kit is suitable for the measurement and analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.

View Megazyme’s latest Guide for Dietary Fiber Analysis.

See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

Two separate methods are described in the associated data booklet:

METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.

Content:	100 assays / 200 assays
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Dietary Fiber
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Signal Response:	Increase
Limit of Detection:	0.5 g/100 g
Total Assay Time:	~ 100 min
Application examples:	Food ingredients, food products and other materials.
Method recognition:	AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19 and CODEX Method Type I