牛胶原蛋白溶液Type I, 3 mg/ml

英文名:Bovine Collagen Solution

货号:BM-5005-100ML

规格:100 ml

品牌: Advanced Biomatrix

报价:5800.00

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商品描述

PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.

Purecol胶原蛋白被称为标准纯度的胶原蛋白(>99.9%胶原蛋白含量),其功能可媲美大多数的原生胶原蛋白。Purecol提取于来自美国封闭式牛群的牛皮,先进的生物生产过程符合严格的质量标准,已被证明是质量超凡的。Purecol胶原蛋白是由约97%的I型胶原蛋白,其余的为III型胶原蛋白组成的,它具有很高的单体含量可以用于测试凝胶渗透色谱。本产品的浓度约为3mg/ml,每个批次的具体浓度会写于产品的COA上随货发出。PureCol是溶于0.01N盐酸的端胶原蛋白,因此它的PH值约为2。PureCol胶原蛋白是理想的蛋白质涂层,可用于准备培养的细胞或者固体凝胶。PureCol胶原蛋白的包装便于储存和使用,经过灭菌的溶液随时可以使用。
 

 

产品信息
PureCol® 胶原蛋白溶液
货号 # BM-5005-100ML
灭菌方法
过滤
形态
溶液
包装尺寸
100 ml
储存温度
2-10 °C
有效期
详见产品包装与随货发出的COA
浓度(缩二脲蛋白测定)
2.9-3.2 mg/ml
纯度(胶原蛋白浓度)
> 99.9%
pH
pH约为2, 0.01 N HCl
凝胶时间(凝胶时间测定法)
< 40 分钟
原纤维形成(纤维形成)
>0.5 吸光度单位
聚丙烯酰胺凝胶电泳(SDS–PAGE)
> 85%含胶原蛋白在α,β,和γ带,< 15%含比α带快的胶原蛋白
无菌的
没有细菌生长
内毒素 (LAL)
< 1.0 EU/ml
渗透压
~ 20 mOsm
电导率
~ 2300 µs/cm
细胞附着实验
通过
来源
牛皮–胃蛋白酶提取

使用方法:

 

Coating Procedure

 

Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
  2. Dilute PureCol® in water to ~50 to 100 µg/ml (~1:30). A 0.01 M HCl solution may also be used.
  3. Swirl contents gently until material is completely mixed.
  4. Add appropriate amount of diluted PureCol® material to the culture surface ensuring that the entire surface is coated.
  5. Incubate at room temperature or 37°C, covered, for 1-2 hours.
  6. After incubation, aspirate any remaining material.
  7. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
  8. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

3-D Gel Preparation Procedure

  1. Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
  2. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
  3. Adjust final volume to a total of 10 parts with sterile water.
  4. To prevent gelation, maintain temperature of mixture at 2–10°C.
  5. To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.

 
Without UltraPure® With UltraPure®
Human Umbilical Vein Endothelial Cells (HUVEC) 10X
VERO Epithelial Cells 10X
Human Dermal Fibroblast Cells 10X
MDCK Epithelial Cells 10X

注意事项:

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
 

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