L-谷氨酸/氨检测试剂盒[快速]

英文名:L-Glutamine/Ammonia (Rapid) Assay Kit

货号:MK-GLNAM

规格:100 assays – 50 of each component

报价:2816.00

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商品描述

The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of L-Glutamine and Ammonia
in cell culture media, foodstuffs and other materials

Principle:
(glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+

(microbial glutamate dehydrogenase)
(2) NH4+ + 2-Oxoglutarate + NADPH → L-glutamate + NADP+ + H2O

Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Cell culture media and cultures, dietary supplements, vegetables
and other materials (e.g. biological samples, etc.)
Method recognition: Novel method

Advantages

  • Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
  • All enzymes supplied as stabilised suspensions
  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

文档

EK-GLNAM Data Booklet.pdf:
EK-GLNAM FAQ.pdf:
m.catalog.equl.cn.pdf:

视频

Sample Pre-treatment Format: