内切纤维素酶检测试剂盒(CELLG3方法)

英文名:Cellulase Assay Kit (CELLG3 Method)

货号:MK-CELLG3

规格:180 / 360 assays per kit / 720 (auto-analyser)

报价:5040.00

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商品描述

For the specific measurement of cellulase (endo-1,4-β-glucanase) in enzyme preparations and fermentation products.

Colourimetric method for the determination of endo-1,4-β-
glucanase (cellulase) in enzyme preparations and fermentation
products

Principle:
(endo-1,4-β-glucanase)
(1) Benzylidene-G3-β-CNP + H2O → Blocked-GX + G(3-X)-β-CNP

(thermostable β-glucosidase)
(2) G(3-X)-β-CNP + H2O → D-glucose + CNP

(alkaline solution)
(3) CNP → phenolate ion (yellow colour)
Note: CNP = 2-Chloro-4-nitrophenol

Kit size: 180 / 360 assays / 720 (auto-analyser)
Method: Spectrophotometric at 400 nm
Total assay time: ~ 20 min
Detection lim-it: 0.05 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition: Novel method
Note:
The analogous fluorimetric reagent (R-CELLF)
is also available with 10-fold greater sensitivity

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested
  • Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6
  • Simple format. Well suited to automation
  • Standard included

文档

K-CELLG3_1301_DATA.pdf:
m.catalog.equl.cn.pdf:

视频

Sample Pre-treatment Format: