L-天冬酰胺/ L-谷氨酰胺/氨检测试剂盒(快速)

英文名:L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid)

货号:MK-ASNAM

规格:100 assays (50 of each) per kit

报价:3008.00

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商品描述

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of L-Asparagine, L-Glutamine 
and Ammonia in potatoes, foodstuffs and cell culture media

Principle:
                               (glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+

                             (microbial glutamate dehydrogenase)
(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O

                                (asparaginase)
(3) L-Asparagine + H2O → L-aspartate + NH4+

Kit size:                              50 assays of each
Method:                              Spectrophotometric at 340 nm
Reaction time:                   ~ 20 min
Detection limit:                  0.50 mg/L (L-asparagine)
                                           0.54 mg/L (L-glutamine)
                                           0.06 mg/L (ammonia)
Application examples:
Potatoes, potato products, vegetables, cereals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:          Novel method

Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
     
  • All enzymes supplied as stabilised suspensions
     
  • Only kit available
     
  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

文档

K-ASNAM_DATA.pdf:
m.catalog.equl.cn.pdf:

视频

Sample Pre-treatment Format: