α淀粉酶SD检测试剂盒(高感光度法)

英文名:α-Amylase SD Assay Kit

货号:MK-AMYLSD

规格:160 / 320 assays (manual) / 640 assays (auto-analyser)

报价:4080.00

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数量:

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商品描述

The Amylase SD Method: A highly sensitive colourimetric method for the determination of α-amylase in sprout damaged wheat grain (also known as pre-harvest sprouting or weather damaged wheat grain) and “late maturity α-amylase” wheat grain.

Advantages

  • Extremely high sensitivity - 2.4-fold increase over Ceralpha (K-CERA)
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

  • Suitable for Maual and auto-analyser formats

Highly sensitive colourimetric method for the determination of α-Amylase in sprout damaged grain

Principle:
(α-amylase)
(1) Ethylidene-G7-α-PNP + H2O → Ethylidene-GX + G(7-X)-α-PNP

(thermostable α-glucosidase)
(2) G(7-X)-α-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size: 160 / 320 assays (manual) / 640 (auto-analyser)
Method: Spectrophotometric at 400 nm
Total assay time: ~ 5 min
Detection limit: 0.05 U/mL
Application examples: Sprout damaged wheat grain
Method recognition: Novel method

文档

K-AMYLSD_DATA.pdf:
m.catalog.equl.cn.pdf:

视频

Sample Pre-treatment Format:

Amylose Amylopectin Assay Procedure: